Flight qualification of ion thrusters typically requires testing on the order of 10,000 hours. Extensive knowledge of wear mechanisms and rates is necessary to establish design confidence prior to long duration tests. Consequently, real-time erosion rate measurements offer the potential both to reduce development costs and to enhance knowledge of the dependency of component wear on operating conditions. Several previous studies have used laser induced fluorescence (LIF) to measure real-time, in situ erosion rates of ion thruster accelerator grids. Those studies provided only relative measurements of the erosion rate. In the present investigation, a molybdenum tube was resistively heated such that the evaporation rate yielded densities within the tube on the order of those expected from accelerator grid erosion. A pulsed UV laser was used to pump the ground state molybdenum at 345.64nm, and the non-resonant fluorescence at 550-nm was collected using a bandpass filter and a photomultiplier tube or intensified CCD array. The sensitivity of the fluorescence was evaluated to determine the limitations of the calibration technique. The suitability of the diagnostic calibration technique was assessed for application to ion engine erosion rate measurements.